Understanding of hsa-mir-654-3p role in Lung Cancer process by bioinformatics analysis
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Fatemeh Abedi dorcheh,1,* Mohammad Bagheri,2
1. Department of Biochemistry, School of Bioscience and Biotechnology, Shahid Ashrafi University of Sepahanshahr, Isfahan, Iran
2. Department of Biological Sciences and Biotechnology , University of Kurdistan , Sanandaj , Kurdistan, Iran
- Introduction: Lung Cancer is the leading cause of years of life lost because of cancer and is associated with the highest economic burden relative to other tumor types. Early diagnosis and treatment has a significant role in the progression and development of cancer. There are many different factors in the prevention, diagnosis and treatment of Lung cancer. Recently, it has been shown that small non-coding RNAs like Micro-RNAs can be used to treat or diagnose various diseases. Therefore, in this bioinformatics study has been used enrichment analysis method to evaluated the role of hsa-mir-654-3p in Lung Cancer. Survey the genome's variation has direct relationship with identifying and diagnosing different types of cancer. By controlling the gene expression, micro-RNAs can play an important role in inhibition of cancers. Consequently, the occurrence of various mutations can lead to change the efficiency of micro-RNAs action.
- Methods: First, the information of hsa-miR-654-3p extracted from the miRBase database. Then, the target genes of hsa-mir-654-3p were obtained using miRTarbase, miRWalk, TargetScan and DIANA TOOLS databases, respectively. Enrichment analysis of microRNA's was done by David's database. By using GraphPad Prsim 3.8.0 , databases and R a programming language, were selected to obtain essential data about microRNA basis. Using the Genemania database, the interactions between the key genes of this study was obtained. Afterwards And then the effect of SNP on microRNA's attachment tendency, gained from miRSNPdb, miRdSNP and miRNASNP database.
- Results: According to the study, it was determined that hsa-mir-654 by affecting the genes ERK, Cycline D1, K-ras, FOXO3, PKC in "MAPK signaling pathway, ErbB signaling pathway, Ras signaling pathway, PI3K-Akt, Calcium signaling pathway" may have role of Lung Cancer. Also, rs1063054 in NBN gene have recognized as a potential biomarker in Lung Cancer. Genes' expression analysis via heatmap method, showed that K-ras gene of hsa-mir-654-3p, probably assigns the largest difference in its expression among solid tissue normal and primary solid tumor.
- Conclusion: Analysis of MAPK, ErbB, Ras, PI3K-Akt and Calcium signaling pathway showed proliferation and anti apoptosis role in Lung Cancer via the activated of ERK, Cycline D1, K-ras, FOXO3, PKC genes which they are activated by a cascade of genes. Thus, the function prevention of mentioned genes results oncogenic role of hsa-mir-654-3p in Lung Cancer. For a better understanding of the hsa-mir-654-3p role in Lung Cancer, the investigation of rs1063054 role in this cancer, is needed.
- Keywords: Bioinformatics, miRNA, SNP, Lung Cancer, Signaling pathways
