Evaluate the immune properties of marine-based Collagen dressing in wound care management
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Bahare Padekan,1,* Mohammadreza Mobayen,2
1. Burn and Regenerative Medicine Research center, Guilan University of Medical Sciences, Rasht, Iran
2. Department of surgery, school of medicine Poursina hospital, Guilan University of medical sciences, Rasht, Iran
- Introduction: In recent years wound care products attracted much research’s attention, intending to replicate or add to the extracellular matrix (ECM), provide more patients assent, reduce healing time and costs. Wound healing consists of sequence processes: hemostasis, inflammation, proliferation, and remodeling. In delayed wound healing, the healing process stopped at one of these phases, especially at inflammation due to the prolonged existence of a high level of metalloproteinase (MMPs), which destructed healthy ECM and prevented re-epithelialization. The ECM is the major component of the dermis, which provides proteins, enzymes, growth factors, and the dynamic structure for cells that are essential for wound healing. Collagen is the abundant fibrous protein in mammalian bodies, and that is the main component in the extracellular matrix (ECM).
Collagen plays an important role, in all wound healing processes, by attracting fibroblasts formation, increasing keratinocyte cells migration, and collagen deposition in the wound bed. There are many different sources for collagen extraction. But among these, fish waste is a low-cost and safe source for collagen extraction. Fishery processing provides 50% to 70% fishery waste with protein bases, which causes several environmental problems. In this article, we aimed to investigate the immune properties of marine-based collagen as a bioactive component for wound care management products.
- Methods: In order to investigate the immune properties, the marine-based collagen was purchased from the Rozhan Vista Mehr Ltd group, and immune properties were evaluated by studying the in-vitro cytotoxicity test MTT, the test for in-vivo irritation, and skin sensitization. All of the examinations were conducted by Nikopharmed Arya Co laboratory.
- Results: The in-vitro cytotoxicity response in L929 mouse fibroblast NCTC for the marine-based collagen showed 95.51% viability, and No reactivity in morphological grade for 24 hours. The results provide evidence to support that the Collagen, Type I is Non-Toxic. The irritation response category in a rabbit of the marine-based collagen for 72±2 hours was 0, so the mean score is Negligible. The results provide evidence to support that the Collagen, Type I is Non-Irritating. The sensitization response category in Guinea pigs of the marine-based collagen for 48±2 hours was 0, so the mean score is No visible change. The results provide evidence to support that the Collagen, Type I is Non-Sensitizing.
- Conclusion: As a result, marine-based collagen demonstrated unique properties such as non-toxicity, non-irritating, and non-sensitizing, consequently the best choice as a bioactive component for wound care management and biomedical applications.
- Keywords: Collagen, wound healing, fish waste, biomedical, immune properties
