Evaluation of the effects of synthesized nano-niosomes containing Artemisia turcomanica extract on the expression of caspase3 gene in breast cancer cell lines (MCF-7)
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Seyedeh Narges Naemi,1,* Zahra Keshtmand1,2 Ardeshir Hesampour,3
1. Departmant of Biology,Central Tehran Branch, Islamic Azad Univercity, Tehran, Iran
2. Departmant of Biology,Central Tehran Branch, Islamic Azad Univercity, Tehran, Iran
3. Departmant of Biology,Central Tehran Branch, Islamic Azad Univercity, Tehran, Iran
- Introduction: Nanoniosomes have been considered as carriers for targeted drug delivery in modern drug delivery systems. Considering the unique biological properties of Artemisia turcomanica plant.The aim of this study was to investigate the effects of nano- niosomes containing Artemisia turcomanica extract on the expression of caspase3 gene in breast cancer cell lines MCF-7.
- Methods: In this experimental study, Artemisia turcomanica plant extract was prepared and nano-niosomes thin film was fabricated using the film method and Artemisia turcomanica plant extract was loaded with different concentrations. Physicochemical properties of nano-niosomes by SEM, FTIR and DLS approved. The cytotoxic effects nano- Niosomes containing extracts on MCF-7 cell line at concentrations of 1000, 500, 250, 125, 62.5, 31.25, 15.625μg/ml were investigated by MTT method. Subsequently, also the expression of caspase 3 gene of MCF-7 cells at concentration of IC50, was investigated using Real Time PCR.
- Results: The results of this study showed that the synthesized nano-niosomes containing the extract have a spherical structure and its size is less than 30-50 nm. Treatment of MCF-7 cells with nano-niosomes containing different concentrations of extracts for 24 hours at concentrations of 1000 and 500 μg/ml had the highest inhibition of cell proliferation which was statistically significant (P<0.001).It was also found that the concentration of IC50 nano-niosomes containing the extract (230.56µg/ml.) reduces the expression of caspase 3 gene in breast cancer cell line MCF-7.
- Conclusion: According to the results, it is possible that in the future, nano-niosomes can be used as a candidate drug combination for pharmaceutical purposes, but more studies are needed.
- Keywords: Artemisia turcomanica, nano-niosome , Caspase 3, Breast Cancer Cell line MCF-7.
