An investigation of rs7903146 polymorphism in TCF7L2 gene in patients with type 2 diabetes mellitus
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Salar Momen Maragheh,1,* Fahimeh Saeifar,2
1. Islamic Azad University of Tehran
2. Health Center of Maragheh
- Introduction: The rs7903146 polymorphism in the TCF7L2 gene is
the most common polymorphism associated with type 2 diabetes mellitus. The aim
of this study was to investigate the association between rs7903146 polymorphism
in the TCF7L2 gene and type 2 diabetes mellitus in the population of Eest
Azerbaijan Province, Iran.
- Methods: A number of 101 blood samples were collected from
patients with diabetes mellitus and 101 from healthy individuals. PCR and
electrophoresis were performed after DNA extraction and quality assay of all
samples with specific primer. Finally, PCR products were treated with Rsal
restriction enzyme and re-electrophoresed and the target polymorphism was
investigated. Finally, PCR products were treated with Rsal restriction enzyme and
re-electrophoresed and the target polymorphism was investigated.
- Results: The frequency of genotypes was calculated as TT = 33.7, CC = 16.8 and CT
= 49.5 (percent) for healthy individuals and TT = 43, CC = 14 and TC = 43 (percent)
for patients. The percentage of T allele in healthy individuals and patients was
42.1% and 64.5%, and the percentage of C allele in healthy individuals and patients
was 57.9% and 35.5%, respectively.
- Conclusion: There is possibly an association between the rs7903146 polymorphism
in the TCF7L2 gene and the risk of diabetes mellitus. Therefore, further studies in
this field can provide more accurate results and examine the diagnosis of the
possibility of developing diabetes in different populations.
- Keywords: polymorphism, rs7903146, TCF7L2, type 2 diabetes.
