Immunogenicity of the combination of two outer membrane proteins, Oma 87 and BauA against Acinetobacter baumannii infection in a murine model
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IRAJ RASOOLI,1,* MASOUMEH SADEGHPOOR,2
1. SHAHED UNIVERSITY
2. SHAHED UNIVERSITY
- Introduction: Acinetobacter baumannii is a non-motile multiform pleomorphic Gramnegative cocco bacillus.Acinetobacter baumannii is a mortal nosocomial pathogen. Resistant to disinfectants, desiccation, and biofilm formation on the abiotic surfaces, and desiccation make A. baumannii as a prosperous successful pathogen in hospital environments. Acinetobacter baumannii has happen become a tremendous challenge to late modern healthcare system healthcare as an antimicrobial resistant. Outer membrane proteins (OMPs) of gram-negative bacteria are known as powerful strong immunogens. Siderophore molecules and Iron-Regulated Outer Membrane Proteins (IROMPs) are the two necessary essential members of iron attainment acquisition system. Siderophores are secreted by bacteria to stick bind circumferential peripheral ferric iron and the IROMPs are expressed at the bacterial outer membrane as the receptor of ferric-siderophore assembled complex.
- Methods: BauA is the corresponding siderophore receptor of A. baumannii. In addition to antibacterial and opsonizing activity, monoclonal antibodies produced against IROMP can also block the in vitro iron absorption system. Antibodies against BauA functional regions can potentially block its functions and lead to impaired iron absorption. Therefore, it makes sense to use bioinformatics tools to select the appropriate area (s) as a vaccine candidate (s). Oma87 has been stated introduced as an immunogenic outer membrane protein per via contrary reverse vaccinology. The going current investigation research undertakes a perusal study on the immunogenicity of recombinant Oma87 in a murine sample model. BAM(Oma87) proteins are another essential OMP. The β-Barrel assembling machine (BAM) creates a multi-protein complex on the outer membrane of gram-negative bacteria that is involved in targeting and folding β-Barrel outer membrane proteins. BamA bears no resemblance to the human and mouse proteome, which is essential for inhibiting autoimmune responses in the host .Active and inactivated vaccination are considered as options for screening against the pathogen A. baumannii. . In this study, both Oma87 and BauA proteins were cloned into the plasmid pET28a. After purification, they were injected in combination to create immunity in mice. After that, the level of IgG in them was evaluated by ELISA method and the established safety was evaluated.
- Results: . In this study, both Oma87 and BauA proteins were cloned into the plasmid pET28a. After purification, they were injected in combination to create immunity in mice. After that, the level of IgG in them was evaluated by ELISA method and the established safety was evaluated. Oma87 and BauA were already repotted to raise antibodies against these proteins.
- Conclusion: The same results were obtained. The combination of the two antigens led to significant protection against A.baumannii in comparison to the single antigens. As a result, the administration of combination antigens provides better protection than individual antigens.
- Keywords: Acinetobacter baumannii,, Vaccine, Oma87, BauA
