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    The Effect of Catechin Encapsulated Chitosan/Alginate Nanoparticles on Memory Impairment

  • Elnaz Mohammadbaghban,1 Ali Taravati,2 Hossein Khaleqzadeh,3 Hossein Najafzadeh,4 Fatemeh Tohidi,6,*
    1. Babol University of Medical Sciences
    2. University of Mazandaran
    3. Babol University of Medical Sciences
    4. Babol University of Medical Sciences
    6. Babol University of Medical Sciences


  • Introduction: Numerous evidence indicated the role of green tea from a traditional beverage to a source of bioactive substances with many health benefits. Green tea is rich in catechin, which mentioned as a polyphenolic compound with favorable properties such as antioxidant, neuroprotective, antitumor, antidiabetic and more others. Albait, catechin promotes the intelligence, memory and strength of the senses, but it removed from circulatory system, rapidly and also it isn’t good enough to act as a therapeutic agent for targeted drug delivery and controlled release in reaching to central nervous system. Due to the unique pharmacology of the catechin, current study designed as a mean of enhancing effectiveness of the catechin at the biochemical and behavioral levels against Aluminum chloride induced Alzheimer’s disease in rat model.
  • Methods: : Ionotropic gelation method as a non-toxic, simple and controllable method was used for synthesis of the chitosan-alginate nanocarriers containing drug catechin. Physio-chemical characterization of the nanoparticles (NPs) has been detected and approved. Sixty male Wistar rats were divided into six groups (one control group and five treatment groups). In order to evaluated the efficacy and protective effect of catechin loaded NPs (10 mg/kg) and catechin (50 mg/kg) against brain injury caused by AlCl3, the spatial memory impairment model was induced by daily injection of AlCl3 (100 mg/kg) intraperitoneally for 21 days. Afterwards, spatial memory and cognitive function were assessed through Morris water maze task. Also, enzymatic assay of acetylcholinesterase (AchE) catalase (CAT) and total antioxidant capacity (TAC) was performed using hippocampal tissue.
  • Results: The results indicated that synthesized NPs had optimal physio-chemical characteristics. AChE activity increased in AlCl3 treated group that confirmed memory impairment (p<0.001). In contrast, improved memory function was seen through the stability of AchE, CAT and TAC levels in the protective groups that received free and encapsulated catechin. Moreover, based on our findings, the gradual improvement of the learning process in the Morris water maze task and the increase in time spent in the target quadrant on probe day was a confirmation of the effective performance of catechin in the protective groups. However, the AlCl3 receiving group showed a statistically significant correlation with increasing the scape latency and decreasing the time spent in the target quadrant compared to the control group and other groups (p<0.05).
  • Conclusion: Present study demonstrated that chitosan-alginate NPs elevated the drug catechin performance and improved the memory function. Hence, this therapeutic approach can be applied effectively and propose new aspects for further researches on dementia paradigms.
  • Keywords: Alzheimer's disease, Catechin, Drug delivery, Chitosan, Alginate