کتابچه خلاصه مقالات همایش


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    Fabrication and characterization of a nanocomposite bacterial cellulose based wound dressing

  • Homa Mohaghegh,1,* Zahra Asadi,2 Elahe Masaeli,3
    1. Department of Biology, ACECR Institute of Higher Education, Isfahan, Iran-Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
    2. unaffiliated
    3. Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran


  • Introduction: Bacterial cellulose (BC) is a natural biosynthetic cellulose hydrogel, mainly produced by well-known Gluconacetobacter xylinus bacteria. BC is being widely explored for various medical applications due to its favorable characteristics such as mechanical properties, biocompatibility, biodegradability, and high moisture content. Although there are many findings on the suitability of BC hydrogel as a wound dressing, BC itself lacks antibacterial properties and is thus unable to provide a barrier against wound infection. Therefore, numerous approaches have been introduced to modify BC and optimize its antimicrobial activity. Integrating a delivery system of antimicrobial agents to BC hydrogel is a promising solution to this problem. Electrospun nanofibrous membranes are recognized as sustainable drug delivery systems, capable of loading a wide range of drugs through a variety of methods. Coating nanofibers with antibiotics is a local and temporary loading method which plays a physical role in protecting the wound, and accelerating healing process by treating infections. In this regard, here, we fabricated a composite wound dressing consisting a BC hydrogel and ciprofloxacin (CIP)-loaded polycaprolactone (PCL) nanofibrous mat to take advantage of all the distinct properties of these materials in wound healing. Nanofibrous PCL mat was chosen because it has been frequently regarded as a highly propitious carrier for loading low-molecular-weight antibacterial agents. CIP is also a fluoroquinolone antibiotic, capable of inhibiting the growth of both gram-positive and gram-negative bacteria in infectious wounds. Unique characteristics of designed nanocomposite dressing like biocompatibility and biodegradability as well as controlled release ability and antibacterial properties, make it a potential applicant for wound healing applications.
  • Methods: BC membrane was produced by culturing Gluconacetobacter Xylinus in Hestrin-Schramm culture mediumو including D-glucose (20g l-1), peptone (5gl-1), yeast extract (5gl-1), Na2HPO4 (2.7gl-1) and citric acid (1.15g l-1) at 30 C° for 7days. Nanofibrous layer was produced by electrospinning of PCL (12%wt) in a composite solvent containing ethanol/chloroform. Immediately after electrospinning, CIP solution was electrosprayed on nanofibrous mat. Finally, CIP-PCL layer was positioned on BC hydrogel to obtain a nanocomposite wound dressing. Successful loading of CIP on PCL fibers was assessed through Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) spectroscopy. Subsequently, wettability and rate of water vapor movement through the samples was determined. Finally, the antibacterial assessment study was carried out using both gram-negative (Escherichia coli or E-coli) and gram-positive (Staphylococcus aureus or S. aureus) bacteria.
  • Results: The ATR-IR spectrum of PCL/CIP scaffold demonstrated a characteristic peak around 1605m-1 and 3109-3155cm-1 correlated to NH group and aromatic ring of CIP, respectively. Water contact angle and rate of water vapor movement of drug-loaded dressing were measured in the range of 25-30° and 23-24mg/cm2.h, respectively. Antibacterial activity also indicated a clear zone of inhibition around the drug-loaded nanofibrous mat against both Gram-positive and Gram-negative bacteria, while no antibacterial activity was detected for drug-free dressing.
  • Conclusion: To conclude, loading and deposition of CIP on the surface of PCL nanofibrous membrane has been successfully developed and a drug-delivery system with suitable hydrophilicity and moisture permeability obtained. Composition of CIP-PCL layer and BC hydrogel resulted a modified nanocomposite dressing that exhibited significant antibacterial rates with more than 99% reduction against E. coli and S. aureus. In summary, obtained results manifest that nanocomposite CIP-PCL/BC membrane is promising for antimicrobial wound dressing with good biocompatibility to promote wound healing.
  • Keywords: Bacterial cellulose; Polycaprolactone; Ciprofloxacin; Wound dressing.