کتابچه خلاصه مقالات همایش


دانلود کتابچه

    Bioinformatics study of the effect of FHIT gene on colorectal cancer cells

  • Ali Attaripour Isfahani,1 Reza Ghanbari,2 Mansoureh Azadeh,3,*
    1. Department of Biological Science and Technology, Najafabad Branch, Islamic Azad University, Najafabad, Iran
    2. Department of Biological Science and Technology, Najafabad Branch, Islamic Azad University, Najafabad, Iran
    3. Zist-fanavari Novin Biotechnology Department, State Technical and Vocational Training Organization, Isfahan, Iran


  • Introduction: Caretaker genes, ensure genome stability as a tumor suppressor gene (TSG). Fragile Histidine Triad diadenosine Triphosphatase (FHIT), which is in the same group of caring genes, usually preserves DNA and prevents its decomposition. In this study, using Bioinformatics databases, effective signaling pathways on colorectal cancer cell apoptosis and its relevance with the FHIT gene has been investigated.
  • Methods: In this bioinformatic study, the GEO database was used to determine the chromosomal profile, target genes and validation of the FHIT gene as a tumor suppressor gene. Complete statistics including the position, function and Expansion of the FHIT gene in distinct organs, particularly the colon was obtained by using UniGene and GeneCards database. Subsequently, the DAVID database was used to genetic pathways evaluation.
  • Results: The FHIT gene is most abundant in the plasma membrane and is involved in the translation of 25 types of proteins. Protein encoded by this gene implicated in purine metabolism. This gene encompasses the fragile site FRA3B on chromosome 3p14.2, where carcinogenic damage may cause aberrant transcripts. FHIT has the function of inducing apoptosis of colorectal cancer cells apoptosis via SRC and AKT1 signaling pathways. FHIT also manages apoptosis with the aid of three main AMD1, SRC, MDM2 genes. In this way AMD1 by encoding "S-adenosylmethionine decarboxylase proenzyme" repairs stem cells, SRC through Translation "Proto-oncogene tyrosine-protein kinase Src" controls apoptosis and tumor migration and ultimately inhibits MDM2-mediated proteasomal degradation of p53/TP53.
  • Conclusion: Present study indicates that the FHIT gene performs a main position in signaling the apoptosis pathway of colon cancer cells, while its deficiency or inefficiency lead to carcinogenic mutations in the colon tissue. As a result, the FHIT gene has impressive potential for designing colorectal cancer modern treatments.
  • Keywords: Colorectal cancer, FHIT gene, Tumor suppressor gene, Apoptosis