Evaluation of insulin expression and toxicity of hybrid nano-liposomal extract in diabetic RINm-5F cell line
-
Manigeh Pouladian,1,* Mina ramezani,2 Zeinab piravar,3
1. Rafsanjani University Campus, Islamic Azad University Central Tehran Branch
2. Rafsanjani University Campus, Islamic Azad University Central Tehran Branch
3. Rafsanjani University Campus, Islamic Azad University Central Tehran Branch
- Introduction: Type 2 diabetes is a common metabolic disorder. Each year, 170 million people worldwide develop diabetes, 95% of which is related to type 2 diabetes. In this study, the effect of combined nanoliposomal plant extract (green tea, chicory and garlic) with metformin (a common type 2 antidiabetic drug) on insulin expression was compared.
- Methods: Thin layer watering method was used to prepare nanoliposome structures. RINm-5F cells, which are beta cells of mouse pancreas, were obtained from the Iranian Genetic Resources Center and propagated in RPMI 1640 medium. The cells were then diabeticized with streptozotocin (STZ) and the diabetic cells were divided into 5 groups. Three groups were treated with different concentrations of combined nanoliposomal plant extracts (0.4, 1 and 2 mg / ml) for 48 hours, one positive control group receiving the anti-diabetic drug metformin at a concentration of 5 mg / ml for 72 hours. And a negative control group that were treated only with FBS. MTT test was used to evaluate the toxicity of plant extracts and then the level of insulin expression in different groups was compared by immunohistochemical test.
- Results: The morphology of the cells changed after treatment with metformin or extract, especially at higher doses of the extract, and changed from epithelioid and cubic to spindle and elongated forms, which indicates more proliferation and adhesion of cells to the bottom of the culture flask. MTT test showed that the combined nanoliposomal plant extract had no toxicity to the cells and did not reduce the cell viability percentage compared to the control group. Also, the results of immunohistochemical test showed that plant extract at a concentration of 2 mg / ml significantly increased insulin expression compared to metformin (P <"0.01").
- Conclusion: The results of toxicity test and insulin production showed that the combined nanoliposomal extract of the plant green tea, chicory and garlic is useful for the treatment of type 2 diabetes because, despite being less toxic, it is more effective at a concentration of 2 mg / ml compared to metformin. .
- Keywords: Diabetes, nanoliposomes, plant extracts, metformin
