Bioinformatics study of genetic pathways influenced by miR-146a in cancer incidence
-
Reza Ghanbari,1 Mohammad Niazi,2 Mohsen Hosseini,3 Mohammad Sadeghi,4 Shabnam Kermani,5,*
1. Department of Biological Science and Technology, Najafabad Branch, Islamic Azad University, Najafabad, Iran
2. Department of Biological Science and Technology, Najafabad Branch, Islamic Azad University, Najafabad, Iran
3. Department of Biological Science and Technology, Najafabad Branch, Islamic Azad University, Najafabad, Iran
4. Department of Biological Science and Technology, Najafabad Branch, Islamic Azad University, Najafabad, Iran
5. Department of Biological Science and Technology, Najafabad Branch, Islamic Azad University, Najafabad, Iran
- Introduction: MicroRNAs are small non-coding molecules, which play regulatory roles in post-transcriptional gene expression. In this study, miR-146a genetic pathways were investigated in different types of cancer by using bioinformatics databases.
- Methods: Focusing on bioinformatics aspects, miRbase and GEO databases were used to determine chromosomal profile and target genes of miR-146a. MiR-146a subcellular location and its level of expression in different organs in a healthy and tumorous state were evaluated in the GeneCards and GEPIA2 database. Finally, the DAVID database was used for genetic pathways analysis.
- Results: MiR-146a gene is located on chromosome 5q33.3. This gene is expressed in different organs and cell components and it plays a regulatory role in the post-transcriptional pathway of 1442 predicted genes. Furthermore, among these gene expression pathways, by mutations in 106 cases such as APC, Ki-ras, E2F, BCL2, and WNT genes, a variety of cancers can be caused, including Endometrial cancer, Bladder cancer and Prostate Cancer. It was also identified that miR-146a plays an important role in signaling pathways such as P53, ErbB, Ras, TNF.
- Conclusion: Current study reveals that miR-146a plays a regulatory role in post-transcriptional pathway of a large number of genes. It also plays an important role in some intracellular signaling pathways.
- Keywords: Pathways in cancer, miR-146a, Intracellular signaling pathways
