کتابچه خلاصه مقالات همایش


دانلود کتابچه

    Investigating the role of hsa-miR-1256-5p as therapeutic targets involved in PI3K-Akt-mTOR pathway in coronary heart disease

  • Alireza Nasr Esfahani,1,* Setare Samizade,2
    1. Department of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
    2. Department of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran


  • Introduction: Coronary heart disease (CHD), which is known as coronary artery disease (CAD), is caused by the buildup of plaque in the arteries that supply oxygen-rich blood to the heart which is one of the main causes of hospitalization worldwide and has high morbidity. NcrRNAs (miRNAs) play an important role in the pathogenesis of cardiovascular diseases that can regulate protein translation by either complete or incomplete pairing with the target gene, or by inhibiting expression of downstream target proteins. MiRNAs have emerged as a novel class of gene regulatory elements with conserved roles in development and disease .So this study purposes to evaluate the association between hsa-miR-1256-5p and PI3K-Akt-mTOR pathway.
  • Methods: Specifications of miRNAs were obtained through mirbase, HMDD and miRdSNP. To identifying target genes, miRTarBase, MIRWALK2.0, TargetScan, DIANA Tools were used. Venn diagram used to identify common target genes between MiRNAs. The signaling pathways for target genes which had high expression difference were observed from the DAVID database and the pathways associated with CHD were stored for interpretation. The network of genes was obtained from GENE MANIA.
  • Results: The result demonstrated the expression levels of related proteins in the PI3K-Akt-mTOR pathway in which hsa-miR-1256 -5p prevents cell growth and proliferation by activating PETEN which blocks AKT. In other side of the pathway mentioned microRNAs inhibit MTOR by blocking PI3KACT and AKT and effect on adhesion increasing by preventing cell survival and proliferation by blocking BRAF and RAS which active MEK1/2, ERK1/2 , RAF and BCL2 through phosphorylation.
  • Conclusion: In this study we found that the role of hsa-miR-1256-5p in CHD is associated with the PI3K-Akt-mTOR pathway in whic hsa-miR-1256-5P might be a novel bio-marker for coronary heart disease. PI3K/Akt pathway is involved in the occurrence and development of vascular physiology and pathology which plays a vital role in the occurrence and development of CHD. Activating the PI3K/Akt signaling pathway can reduce reactive oxygen species (ROS) and lipid deposition levels, inhibit plaque formation, and reverse the progression of CHD.
  • Keywords: Coronary heart disease, Signaling pathways, MicroRNA, target genes