Investigating the role of hsa-miR-1256-5p as therapeutic targets involved in PI3K-Akt-mTOR pathway in coronary heart disease
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Alireza Nasr Esfahani,1,* Setare Samizade,2
1. Department of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2. Department of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
- Introduction: Coronary heart disease (CHD), which is known as coronary artery disease
(CAD), is caused by the buildup of plaque in the arteries that supply oxygen-rich blood to the
heart which is one of the main causes of hospitalization worldwide and has high morbidity.
NcrRNAs (miRNAs) play an important role in the pathogenesis of cardiovascular diseases that
can regulate protein translation by either complete or incomplete pairing with the target gene,
or by inhibiting expression of downstream target proteins. MiRNAs have emerged as a novel
class of gene regulatory elements with conserved roles in development and disease .So this
study purposes to evaluate the association between hsa-miR-1256-5p and PI3K-Akt-mTOR
pathway.
- Methods: Specifications of miRNAs were obtained through mirbase, HMDD and
miRdSNP. To identifying target genes, miRTarBase, MIRWALK2.0, TargetScan, DIANA
Tools were used. Venn diagram used to identify common target genes between MiRNAs. The
signaling pathways for target genes which had high expression difference were observed from
the DAVID database and the pathways associated with CHD were stored for interpretation.
The network of genes was obtained from GENE MANIA.
- Results: The result demonstrated
the expression levels of related proteins in the PI3K-Akt-mTOR pathway in which hsa-miR-1256 -5p
prevents cell growth and proliferation by activating PETEN which blocks AKT. In other side
of the pathway mentioned microRNAs inhibit MTOR by blocking PI3KACT and AKT and
effect on adhesion increasing by preventing cell survival and proliferation by blocking BRAF
and RAS which active MEK1/2, ERK1/2 , RAF and BCL2 through phosphorylation.
- Conclusion: In this study we found that the role of hsa-miR-1256-5p in CHD is associated
with the PI3K-Akt-mTOR pathway in whic hsa-miR-1256-5P might be a novel bio-marker for
coronary heart disease. PI3K/Akt pathway is involved in the occurrence and development of
vascular physiology and pathology which plays a vital role in the occurrence and development
of CHD. Activating the PI3K/Akt signaling pathway can reduce reactive oxygen species (ROS)
and lipid deposition levels, inhibit plaque formation, and reverse the progression of CHD.
- Keywords: Coronary heart disease, Signaling pathways, MicroRNA, target genes