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    Investigation Ashwagandha on human breast cancer cell line by Meta analysis vision

  • Yasaman Najafi,1,*
    1. Department of Biology , School of Science , Razi University,Kermanshah,Iran


  • Introduction: Cancer treatment with herbal compounds is an option with the minimum side effects. Ashwagandha as a plant is useful for reducing stress, boosting immune system, and it contains Withaferin A, as an inhibitor of angiogenesis. In this study, we showed biological pathways changes in MCF-7 cells after treating by Ashwagandha.
  • Methods: The GSE144235 dataset in NCBI was analyzed with GEO2R, the control group (MCF-7, DMSO) and the test group (MCF-7, Ashwagandha _125mcg/ml) have been considered with p.value less than 0.05 ;then to know biological pathways, gene ontology was performed using DAVID database for the list of genes with logFC greater than 0.6 and also less than -0.6.
  • Results: The results clearly show the difference in the expression of genes between the control and test groups. Genes like CCNB1,CDK4,CDC20 involved in cell division and also genes like CDK1,EGFR2, TP53involved in apoptotic process had less expression in test group comparing to the control group.
  • Conclusion: The gene expression changes in biological pathways like apoptosis and cell division after treating MCF-7 by Ashwagandha shows that it can be effective on breast cancer because some up-regulated genes in cancer decreased their expression after treatment by Ashwagandha. This condition indicates that the expression of test group was close to normal situation and this plant is useful to make medicine for breast cancer.
  • Keywords: Breast cancer; Biological pathways; Ashwagandha; Apoptotic process.