The role of ISH technique in the field of molecular biotechnology
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Tohid Javaheri,1,*
1. Young Researchers and Elites Club, Islamic Azad University, Mashhad Branch, Mashhad, Iran
- Introduction: Changes in the genome and proteome of cells disrupt normal cellular control mechanisms. Using biotechnology methods, these molecular errors can be identified and appropriate therapies selected. In situ hybridization (ISH) is one of these techniques. In situ hybridization (ISH) is a type of hybridization that uses a complementary DNA marker, RNA, or modified nucleic acid strand (probe) to concentrate a specific DNA or RNA sequence in a portion or part of tissue (in situ). DNA ISH can be used in medical diagnosis to assess chromosomal integrity. ISH RNA is used to measure and localize RNAs (mRNAs, lncRNAs, and miRNAs) in tissue sections, cells, and circulating tumor cells (CTCs).
- Methods: In this review study, searches were performed on PubMed, Medline, Google Scholar, Scopus and ISI electronic and scientific databases and valid related articles were searched using the keywords In situ hybridization (ISH) and molecular biotechnology.
- Results: Certainly, using ISH technique, important steps can be taken in the field of medical science and biotechnology of cancer.
- Conclusion: ISH technique has increased the knowledge of researchers about molecular events, so according to the performance of ISH technique can be used in research, diagnosis and early treatment of cancer and other emerging infectious diseases.
- Keywords: In situ hybridization (ISH), molecular biotechnology, diagnosis
