correlation between BP1 and SPL1 and protein phosphorylation in spms(multiple sclerosis)
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armin sharifi,1,* mehran radak,2 hosein mohammadi,3 shahab atefi,4
1. medical science of kermanshah
- Introduction: MS, as a chronic autoimmune disease with unknown etiology, is the most common neurological disorder in young adults. It is characterized by the infiltration of auto-reactive immune cells into the central nervous system (CNS), which leads to inflammation, demyelination, and axonal degeneration. Several studies suggest that genetic, environmental, epigenetic and infectious factors may contribute to the etiology and pathogenesis of MS. This essay survey death ratio reason of SPMS patient.
- Methods: In order to start this study, we used GEO accession number GSE131282. In our work, we focus on patient with less disease duration , then by the use of GEO2R web tool, we analyzed the information. Eventually, for every gene list we removed differentially expressed genes (DEGs) which had p-value more than 0.05 and log 2 FC between ±0.6. protein-protein interaction (PPI) was used to find the proteins that is coded by DEGs, thereby string data base was utilized for this aim. The list of DEGs was deposited in this data base and output, PPI network, was received. To analyze PPI networks, Cytoscape 3.4.0 and Gephi software v 0.9.1 were used to visualization of networks.
- Results: samples of patients and controls were compared to identify DEGs in each disease. The number of DEGs were 801 genes in SPMS which 356 were upregulated and 484 were downregulated. Analysis of modules and their functional annotation in SPMS show G-protein coupled receptor signaling pathway and extracellular matrix organization and etc.
- Conclusion: this essay demonstrated that BP1 and SPL1 play crucial role in mortality of SPMS. positive regulation of gene expression and protein phosphorylation have limited in case of PPMS.
- Keywords: #multiple_sclerosis#SPL1 #BP1
