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    Investigation of a long non-coding RNA through ASD, Breast cancer and prostate cancer

  • Melika Nasr Esfahani,1 Pegah Javid,2 Mansoureh Azadeh,3,*
    1. Zist Fanavari Novin Biotechnology Department, Provincial Technical and Vocational Training organization
    2. Zist Fanavari Novin Biotechnology Department, Provincial Technical and Vocational Training organization
    3. Zist Fanavari Novin Biotechnology Department, Provincial Technical and Vocational Training organization


  • Introduction: Abstract: Background. The retinoblastoma (RB1 or RB) is a tumor suppressor gene which is located on Chromosome 13q.14,2. The Neurobeachin (NBEA) gene is an important factor in Autism Spectrum Disorder (ASD). Phosphatase and tensin homolog (PTEN) is a tumor suppressor gene which is located on Chromosome 10q23,3 and its inactivation is a frequent event in many cancers such as breast and prostate cancers. PTEN is also involved in ASD. P53, PTEN and RB1 are tumor suppressor genes, which are frequently mutated in breast cancer, but new researches have shown that at least one of these three genes must be inactivated for oncogenesis. The co-loss of BRCA2-RB1 can enhance the progress of prostate cancer even in primary phases and drive it to more aggressive one and a possibility of FFL (feed-forward loop) between BRCA2 and RB1 expression in prostate cancer. MicroRNAs (miRNAs) are non-coding RNAs which regulate gene expression. Some of miRNAs are associated with oncogenesis, metastasis and tumor suppressing roles in normal cells that are called oncogenic miRNAs or oncomirs. The aim of this study was finding more related links between our knowledge based on previous researches through bioinformatics databases.
  • Methods: Materials and Methods. General information about RB1, BRCA2, PTEN, NBEA, and their interactions were acquired by previous studies. The miRWalk database was used to find the miRNAs associated with desired genes. The lncRNASNP2 database was applied to achieve the involved long non-coding (lnc) RNAs involved in ASD, breast and prostate cancers. The miRBase database and TAM (Tool for Annotations of Micro RNAs) helped to find more details about miRNAs such as sequencing, annotations, structures, and their related diseases.
  • Results: Results. The bioinformatics predictions showed that has-miR-6870-5p is a common miRNA between RB1 and BRCA2, PTEN, and NBEA genes. The lnc:NONHSAT190283.1 is involved in breast neoplasia, prostrate neoplasia and ASD. The miRBase database predictions revealed that has-miR-21-5p and has-miR-6870-5p can regulate each other. The miRWalk database showed that has-miR-6870-5p is a shared miRNA for RB1, BRCA2, PTEN, and NBEA genes and it can regulate their expression in a same way. The bioinformatics predictions showed the lnc: NONHSAT190283.1 as a common regulator between has-miR-21-5p and has-mir-6870-5p. The data showed Autism Spectrum Disorder, breast and prostate cancers that are linked to lnc: NONHSAT190283.1, has-miR-21-5p and has-miR-6870-5p.
  • Conclusion: Conclusion: According to our bioinformatics data, the common NONHSAT190283.1 between has-miR-21-5p and has-mir-6870-5p, it is possible that there would be a connection between RB1, BRCA2, PTEN and NBEA genes might be used as biomarkers through diagnosis of studied disorders.
  • Keywords: Keywords: Autism, cancer, has-miR-21-5p, has-miR-6870-5p, NONHSAT190283.1