Temur therapy with plant secondary metabolites
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fereshteh rouygar,1,* Shafieh Abdullahzadeh,2 Sholeh Ghollasimood,3
1. Birjand University
2. Birjand University
3. Birjand University
- Introduction: Angiogenesis occurs both in physiological conditions (wound healing, embryonic development, etc.) and in pathological conditions (diseases including cancer). This process plays a key role in the growth and invasion of cancerous tissue.
Plants are rich in a variety of secondary metabolites used in the food, pharmaceutical and chemical industries. Among these plants, we can mention Barijeh and Choyl. The metabolites of these plants affect the release of MMP_2 and finally the expression of VEGF-e gene is reduced, which stops or reduces the angiogenesis process and the size of the tumor itself. However, some of these metabolites are sometimes produced in limited quantities in plants with special properties, such as anti-melanogenesis and antioxidants in saffron. Crocin and picrocrosins are effective substances in stopping angiogenesis that are found in small amounts in saffron plant, so this restriction can be removed by using plant metabolite engineering.
- Methods: Genetic manipulations can be applied to increase the production efficiency of the desired secondary metabolite from a plant, but genetic manipulations are not a suitable system to achieve our goals due to complex and time-consuming processes that often lead to unforeseen results. . Hence gene transfer systems are used.
In this article, an overview of Tobacco H. potivirus has been used to transfer the desired gene due to its biosynthetic enzymes and regulatory factors for plants. The recombinant virus is expressed as it enters the plant and the levels of safranal, crocin and picrocrocin in the plant increase.
- Results: In N. benthamiana due to the optimal conditions of the plant as a biological plant. By increasing the proper temperature, picrocrosin becomes the inactive structure of picrocrosin, ie safranal, which is very effective in anti-angiogenic activity.
- Conclusion: The contents of crocin, picrocrocin increased in fruits and tissues of N. benthamiana treated compared to C. sativus stigma or gardenia SSP.
Therefore, to reduce the size of the tumor, the access of this invasive structure from oxygen and .... can be reduced by preventing angiogenesis. The use of plant secondary metabolites as recombinant drugs is a very effective and harmless method in the treatment of many diseases.
- Keywords: Safranal, secondary metabolites, virus, gene transfer, tobacco
