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    Bioinformatics study of miR-let-7c as a tumor cell biomarker for detection of bladder cancer

  • Reza Ghanbari,1 Ali Attaripour Isfahani,2 Mansoureh Azadeh,3,*
    1. Faculty of Sciences, Najafabad Branch, Islamic Azad University, Najafabad, Isfahan, Iran
    2. Faculty of Sciences, Najafabad Branch, Islamic Azad University, Najafabad, Isfahan, Iran
    3. Zist-fanavari Novin Biotechnology Department, State Technical and Vocational Training Organization, Isfahan, Iran


  • Introduction: MicroRNAs are small non-coding molecules, which plays a regulatory role in post-transcriptional gene expression. In this study, using bioinformatics databases, expression levels, target genes, subcellular location and genetic pathways of miR-let-7c were investigated as a bladder tumor cell biomarker for detection of bladder cancer.
  • Methods: Focusing on bioinformatics aspects, miRbase and GEO databases were used to determine chromosomal profile, target genes and validation of miR-let-7c as a bladder tumor cell biomarker. MiR-let-7c subcellular location and its expression levels in different organs especially the bladder in a healthy and tumorous state were evaluated in the GeneCards and GEPIA2 database. Finally, the DAVID database was used for genetic pathways analysis.
  • Results: MiR-let-7c gene is located on chromosome 21q21.1.The miR-let-7c is overexpressed in bladder cancer, so that it is abundantly expressed in different parts of the cell and plays a role in the post-transcriptional pathway of 2224 predicted genes of cell components. Furthermore, of the 1612 predicted miR-let-7c gene targets, which with mistakenly acting on these genescould lead to variety of diseases, it can cause bladder cancer by effecting on 85 genes. It was also identified that the most important subcellular location of miR-let-7c is in plasma membrane. According to the results, miR-let-7c can be widely found in bladder tumor cells.
  • Conclusion: Current study reveals that miR-let-7c plays a role in the post-transcriptional pathway of a large number of genes. It is also highly expressed in bladder tumor cells. As a result, miR-let-7c has a great potential for clinical diagnosis of bladder cancer.
  • Keywords: Bladder cancer, miR-let-7c, Tumor cell biomarker