The Effect of The Antibiotic Ciprofloxacin on The Growth of Pseudomonas Aeruginosa
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Mostafa Rahimi,1 Mojtaba Mohammadi Roknabadi,2 Rasoul Shokri,3,*
1. Master of Microbiology, Microbiology, Islamic Azad, Zanjan Branch
2. Assistant professor of mycology Department of biology, Zanjan Branch, Islamic Azad University, Zanjan, Iran,
3. Assistant professor of Microbiology Department of biology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
- Introduction: The genus Pseudomonas consists of more than 120 species that are ubiquitous in moist environments such as water and soil ecosystems and pathogenic to plants, animals and humans. Within the Pseudomonas species, P. aeruginosa is most frequently associated with causing human infection; however, it naturally exists in the environment. The bacterium is regarded as an opportunistic pathogen, primarily causing nosocomial infections in immunocompromised patients. P. aeruginosa is rarely associated with causing chronic infections in previously healthy patients, although fatal cases of P. aeruginosa infections in previously healthy people have been reported. Ciprofloxacin is a broad-spectrum fluoroquinolone antibacterial agent. Since its introduction in the 1980s, most Gram-negative bacteria have remained highly susceptible to this agent in vitro; Gram-positive bacteria are generally susceptible or moderately susceptible. Ciprofloxacin attains therapeutic concentrations in most tissues and body fluids. The results of clinical trials with Ciprofloxacin have confirmed its clinical efficacy and low potential for adverse effects. This study aimed to investigate the effect of the antibiotic ciprofloxacin on the growth of Pseudomonas aeruginosa.
- Methods: To investigate the effect of the antibiotic ciprofloxacin on the growth of Pseudomonas aeruginosa, Pseudomonas aeruginosa was prepared and cultured, then the bacterial suspension of Pseudomonas aeruginosa and ciprofloxacin was selected as the antibiotic used. Finally, ciprofloxacin was used to determine the MIC and MBC of Pseudomonas aeruginosa on microdilution.
- Results: The results showed to evaluate the effect of the antibiotic ciprofloxacin on the growth of Pseudomonas aeruginosa, a microdilution method was used to evaluate the effect of the antibiotic ciprofloxacin on Pseudomonas aeruginosa in tubes containing Mueller Hinton Brot. And microbial suspension of ciprofloxacin antibiotic dilutions including (4000, 2000, 1000, 500, 250, 125, 62, 31, 16) ml/µg were prepared. The combined MICs and MBCs were then 16 ml / µg and 31 ml / µg, respectively.
- Conclusion: Finally, the results of a laboratory study showed the effect of the antibiotic ciprofloxacin on the growth of Pseudomonas aeruginosa showed that the growth of Pseudomonas aeruginosa in the tube at a concentration of 16 ml / µg was positive in both cases, in the tube and plate. Also, the growth of Pseudomonas aeruginosa a concentration of 31 ml / µg was positive in plate and, negative in the tube.
- Keywords: Pseudomonas aeruginosa, ciprofloxacin, antibiotic, MIC, MBC
