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    In silico analysis of hsa-miR-21-5p target genes and rs4648298 relation with Colorectal Cancer

  • Fatemeh Abedi dorcheh,1,* Mohammad Bagheri,2
    1. Department of Biotechnology, School of Bioscience and biotechnology , Shahid Ashrafi University of Isfahan , Sepahan shahr, Iran
    2. Department of Biological Sciences and Biotechnology , University of Kurdistan , Sanandaj , Kurdistan, Iran


  • Introduction: Colorectal Cancer (CRC), is the third most common type of cancer in the world. Colorectal Cancer listed as a genetic disease, Based on the researches miRNAs (small non-coding RNAs) and SNPs (single nucleotide polymorphisms) might develop the chance of being infected by Colorectal Cancer. this study has been done in order to diagnose a biomarker for early detection of this cancer. Based on the bioinformatics analysis of this research, hsa-mir-21-5p, which is located on chromosome 17 was selected.
  • Methods: To perform a wide range of bioinformatics analysis miRBase , miRTarbase , miRWalk , TargetScan , DIANA TOOLS, miRSNPdb, miRdSNP, miRNASNP, DAVID, GeneMANIA databases, GraphPad Prsim 8.0 , databases and R a programming language, were selected to obtain essential data about microRNA basis , validated and predicted target genes , genes' expression , SNP, signaling pathways and genes' interaction network.
  • Results: According to the hsa-mir-21-5p targets PI3K, Akt, MSH2, MSH6, TGFBR2, APC and p53 genes are considered as the most regular genes of PI3K, PI3K-Akt, MSI, Wnt and p53 signaling pathways of Colorectal Cancer which their similar pattern is clearly notable. Furthermore, in the bioinformatics analysis of this study, the occurrence of rs4648298 in PTGS2, showed to have a vital role in CDC disease process, but to understand the role of this SNP in CDC a wide range of surveys are required.
  • Conclusion: According to PI3K-Akt signaling pathway, some genes containing Akt, PI3K seem might lead to metastasis via causing cell proliferation, cell cycle. Moreover, in p53 signaling pathway, p53 gene which activates via a cascade of genes, is able to uncontrolled proliferation, increased survival and Genomic instability. Thus, inhibition of these genes by hsa-mir-21-5p that lead to metastasis and proliferation, mentioned miRNA is considered to be tumor suppressive.
  • Keywords: Bioinformatics, miRNA, SNP, CRC, Signaling pathways