Isolation and recognization of protease producing bacterium Basillus sp.SM-7 from Caspian sea
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sadaf mollaaghajanzade,1,* Salman Ahmady Asbchin,2 Ali Taravati,3
1. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
2. Depatment of Microbiology,Faculty of Basic Science,University of Mazandaran,Babolsar,Iran
3. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
- Introduction: Marine enviroments supply a suitable place for natural microbial communities which in turn serve as a potential origin of pharmaceutical and nutriceutical substance.
Proteases are among the most important industerial enzymes Which accounts about 65%from global market for industerial enzymes.
- Methods: In this paper,Screening of the protease-producing Bacteria was carried out by skim milk agar.Enzyme assay was investigated by the colorimetric method and the effect of different paramters such as temperature,pH,different NaCl concentration and different ions on enzyme activity were assesed.detection of the isolates was carried out by biochemical and molecular methods
- Results: A total of 12 strains with proteolytic activity were isolated among which the strain S-7 with higher clear areola on skim milk agar was choosen for extra analysis.
maximum proteolytic activity of the enzyme was observed at 30°c,Ph 10.0 ,and the 3% concentration of salinity. Its biochemistery tests and 16s rRNA gene sequencing showed that it is Basillus sp.SM-7.
- Conclusion: This resault notifies that protease SM-7 can be used at biotechnology incloding detergent industries,meat processing,…..
- Keywords: Key Words: Protease,Caspian sea, proteolytic activity, Isolation,Basillus sp
