Investigation of hsa-miR-136-5p target genes in the main route of tongue cancer by David Analysis
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Ziba Rezvanie Sichanie,1 Adel Rezvanie Sichanie,2 Shadi Omidghaemi,3 Mansoureh Azadeh,4,*
1. Zist-Fanavari Novin Biotechnology Institute, Isfahan, Iran
2. Zist-Fanavari Novin Biotechnology Institute, Isfahan, Iran
3. Zist-Fanavari Novin Biotechnology Institute, Isfahan, Iran
4. Zist-Fanavari Novin Biotechnology Institute, Isfahan, Iran
- Introduction: Tongue cancer is the eighth most prevalent malignancy globally, posing a significant threat to human health. MicroRNAs are a group of small non-coding RNAs that include 21-25 nucleotides. That binding to the sequence in 3’UTR of target mRNA. In recent years, miR-136-5p has received much attention as a tumor suppressor for multiple cancers. This study investigated the expression of target genes hsa-miR-136-5P through the comprehensive web-based David method.
- Methods: By Considering the role of miR-136-5P in tongue cancer was obtained predicted targets of miR-136-5P with the MIMAT0000250 ID from the MiRBase database and inserted in the MiRwalk2.0 database and received a gene list. Finally, we obtained it in the DAVID database to get the miR-136-5P-related pathways and we got the genes in the main pathway that cause tongue cancer.
- Results: The Database for Annotation, Visualization and Integrated Discovery (DAVID, http://david.abcc.ncifcrf.gov/) is an online tool for functional gene classification, which is an essential foundation for high-throughput gene analysis to understand the biological significance of genes. KEGG pathway analyses were conducted using the DAVID online tool; p<0.05 was set as the cutoff point. KEGG signaling pathway data and signaling pathway in tongue cancer show as the most statistical pathway associated with hsa-miR-136-5p targeting and it can be said that hsa-miR-136-5p acts as involved in causes tongue cancer.
- Conclusion: hsa-miR-136-5p effect in cancer signaling pathway and suppress the ADH1B and ADH1C gene expression and prevents the function of some genes, such as Ras, Mek, Raf, and Sos genes. All in all, we conclude that hsa-miR-136-5p may lead to tongue cancer
- Keywords: tongue cancer, hsa-miR-136-5p, MicroRNAs, DAVID database
